9RVQ

Structure of CrtA with betaOG

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-22
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.91840
Spacegroup name	P 1 21 1
Unit cell lengths	68.103, 120.081, 119.550
Unit cell angles	90.00, 102.24, 90.00

Refinement procedure

Resolution	116.830 - 2.075
R-factor	0.2502
Rwork	0.247
R-free	0.30300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.000
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	BUSTER (2.10.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	116.830	2.315
High resolution limit [Å]	2.075	2.075
Number of reflections	54840	2742
<I/σ(I)>	5.8
Completeness [%]	88.2
Redundancy	3.3
CC(1/2)	0.992	0.705

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20mM NaCl 50mM MES pH 5.5 14% PEG350 MME