9RVP

Protein 2A from Theiler's murine encephalomyelitis virus (TMEV) bound to RNA pseudoknot

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-10
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9537
Spacegroup name	C 1 2 1
Unit cell lengths	104.191, 45.931, 65.720
Unit cell angles	90.00, 127.34, 90.00

Refinement procedure

Resolution	52.250 - 1.900
R-factor	0.1948
Rwork	0.193
R-free	0.22950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.831
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.250	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.152	0.728
Rmeas	0.166	0.792
Rpim	0.065	0.308
Number of reflections	19441	1235
<I/σ(I)>	7.1	3.3
Completeness [%]	98.7	98.3
Redundancy	6.5	6.7
CC(1/2)	0.974	0.640

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.9	294	150 nL 2A-RNA complex (7.5 mg/mL, 693 uM) in 10 mM HEPES pH 7.4, 100 mM NaCl, 1 mM MgCl2, 1 mM DTT was mixed with 150 nL 0.2 M ammonium acetate, 0.1 M Bis-Tris pH 5.9, 19% (w/v) PEG 3350.