9RVK
Crystal structure of Aurora-A bound to DBL5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-29 |
| Detector | DECTRIS EIGER2 R 1M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.766, 68.852, 89.226 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.569 - 1.789 |
| Rwork | 0.195 |
| R-free | 0.23830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.787 |
| Data reduction software | xia2 |
| Data scaling software | DIALS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.770 | 1.820 |
| High resolution limit [Å] | 1.789 | 1.790 |
| Number of reflections | 33504 | 1648 |
| <I/σ(I)> | 10.7 | |
| Completeness [%] | 99.3 | |
| Redundancy | 13.6 | |
| CC(1/2) | 1.000 | 0.300 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M TRIS pH 8.5, 0.1M BICINE pH 8.5, 12.5% MPD, 12.5% PEG 1000, 12.5% PEG 3350, 0.03M Sodium fluoride, 0.03M Sodium bromide, 0.03M Sodium iodide. |
