9RVF
Structure of Bgla9 from Anoxybacillus ayderensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-09-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.088, 90.733, 92.103 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.500 - 1.600 |
| Rwork | 0.173 |
| R-free | 0.21380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.239 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.500 | 1.650 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.082 | 1.832 |
| Rmeas | 0.087 | 2.075 |
| Rpim | 0.024 | 0.955 |
| Number of reflections | 61852 | 1856 |
| <I/σ(I)> | 14.7 | 0.3 |
| Completeness [%] | 92.4 | 56.6 |
| Redundancy | 11.2 | 4.5 |
| CC(1/2) | 0.999 | 0.323 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 20%w/v PEG4000, 20%v/v 2-Propanol, 0.1 M Na citrate pH 5.5 |
