9RV5
Crystal structure of SPSB2 SPRY domain in complex with linear TAT peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-02-07 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.470, 90.106, 57.817 |
| Unit cell angles | 90.00, 97.47, 90.00 |
Refinement procedure
| Resolution | 48.410 - 1.750 |
| R-factor | 0.17271 |
| Rwork | 0.171 |
| R-free | 0.19886 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.431 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.410 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.141 | 1.123 |
| Rmeas | 0.152 | 1.216 |
| Rpim | 0.057 | 0.461 |
| Total number of observations | 15833 | |
| Number of reflections | 44886 | 2333 |
| <I/σ(I)> | 8.1 | 1.5 |
| Completeness [%] | 99.4 | |
| Redundancy | 7 | 6.8 |
| CC(1/2) | 0.997 | 0.765 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 22% PEG3350 10% Ethylene glycol, 0.2M Sodium nitrate |
