9RTO
Room temperature crystal structure of the perdeuterated TTR mutant V30M at pH5.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2025-05-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.7293 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 43.703, 65.134, 85.189 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.170 - 1.560 |
| R-factor | 0.1769 |
| Rwork | 0.176 |
| R-free | 0.19800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.086 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.700 | 1.610 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Rmerge | 0.073 | 0.662 |
| Rmeas | 0.076 | 0.689 |
| Rpim | 0.021 | 0.190 |
| Number of reflections | 35449 | 3457 |
| <I/σ(I)> | 23 | 5.2 |
| Completeness [%] | 99.9 | 99.48 |
| Redundancy | 13.34 | 12.97 |
| CC(1/2) | 0.998 | 0.928 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 1.7 M sodium malonate pH5 |
