9RTM
X-ray structure of the rhodamine-binding protein tag (Rho-tag) bound to a tetramethylrhodamine ligand
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-11 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.00001 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.090, 62.350, 96.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.000 - 2.100 |
| R-factor | 0.22 |
| Rwork | 0.195 |
| R-free | 0.21620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.913 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.059 | 0.521 |
| Number of reflections | 22503 | 2884 |
| <I/σ(I)> | 16.7 | 3.4 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 6.5 | 6.9 |
| CC(1/2) | 0.999 | 0.905 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1 M sodium phosphate citrate pH 4.2, 1.6 M sodium dihydrogen phosphate, 0.4 M potassium hydrogen phosphate |
