9RSW

Crystal structure of the human METTL3-METTL14 in complex with compound 4

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-10
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 32 2 1
Unit cell lengths	64.190, 64.190, 227.340
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.820 - 2.560
R-factor	0.2195
Rwork	0.218
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.980
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.467	2.720
High resolution limit [Å]	2.560	2.560
Number of reflections	18242	2821
<I/σ(I)>	10.51
Completeness [%]	99.3
Redundancy	7.88
CC(1/2)	0.997	0.592

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	20% PEG 3350, 0.4M Magnesium acetate