9RSV
Complex of rice blast (Magnaporthe oryzae) effector protein AVR-Pia with the HMA domain of OsHPP09 from rice (Oryza sativa)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9655 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 93.665, 93.665, 72.873 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.830 - 1.650 |
| Rwork | 0.176 |
| R-free | 0.20210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.243 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.13) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.830 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.037 | 0.874 |
| Rmeas | 0.044 | 1.059 |
| Rpim | 0.025 | 0.592 |
| Number of reflections | 23168 | 1125 |
| <I/σ(I)> | 19.2 | 1.9 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.6 | 5.9 |
| CC(1/2) | 0.999 | 0.837 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M MES pH 6.5, 0.2 M ammonium sulphate, 20% (w/v) PEG 4000 |
