9RSR
Cryogenic crystal structure of the TTR mutant V30M at pH5.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-03-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.72932 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 85.392, 43.004, 63.685 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.300 - 1.250 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.20890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.096 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.697 | 1.300 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.055 | 0.643 |
| Rmeas | 0.058 | 0.669 |
| Number of reflections | 64266 | 6135 |
| <I/σ(I)> | 24.3 | 4 |
| Completeness [%] | 98.9 | 95.7 |
| Redundancy | 13.2 | 13.1 |
| CC(1/2) | 0.998 | 0.872 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 2.15 M sodium malonate pH 5 |
