9RSP
Cryogenic crystal structure of the perdeuterated TTR mutant V30M at pH5.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-04-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.7293 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 43.276, 64.258, 85.586 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.330 - 1.610 |
| R-factor | 0.1736 |
| Rwork | 0.172 |
| R-free | 0.20060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.826 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.290 | 1.670 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmerge | 0.049 | 0.359 |
| Rmeas | 0.051 | 0.374 |
| Number of reflections | 31442 | 3047 |
| <I/σ(I)> | 33.7 | |
| Completeness [%] | 99.6 | 98.6 |
| Redundancy | 13.5 | 8.7 |
| CC(1/2) | 0.998 | 0.971 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 1.7 M Sodium Malonate pH 5 |
