9RQU

The C-terminal domain of remorin from Nicotiana benthamiana

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 1 21 1
Unit cell lengths	26.554, 99.134, 128.698
Unit cell angles	90.00, 95.79, 90.00

Refinement procedure

Resolution	78.509 - 2.500
Rwork	0.246
R-free	0.29140
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.088
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	CRANK2
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.510	2.600
High resolution limit [Å]	2.500	2.500
Number of reflections	22997	2588
<I/σ(I)>	13.9
Completeness [%]	99.8
Redundancy	6.8
CC(1/2)	0.999	0.934

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	The best crystals of NbREM1.1-97-208 grew from protein solution concentrated to 11.07mg/ml 0.1M HEPES PH 7.5; 0.2M ammonium acetate; 25% PEG3350 in the presence of 0.5 % w/v n-Octyl-beta-D-glucoside. SeMet crystals grew from the same conditions.