9RQS

Human APE1 in complex with DNA

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-11
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.90931
Spacegroup name	C 1 2 1
Unit cell lengths	70.122, 71.707, 75.274
Unit cell angles	90.00, 110.57, 90.00

Refinement procedure

Resolution	35.838 - 1.700
Rwork	0.193
R-free	0.22790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.706
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.850	1.730
High resolution limit [Å]	1.700	1.700
Number of reflections	38179	2031
<I/σ(I)>	12.7	1.3
Completeness [%]	99.3
Redundancy	6.8
CC(1/2)	0.999	0.876

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.15 M ammonium sulfate, 0.1 M Na HEPES pH 7, 20% w/v PEG 4000