9RPC

HIV-1 capsid (M-group) - native

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-17
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97625
Spacegroup name	P 6
Unit cell lengths	92.460, 92.460, 57.760
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	80.070 - 1.969
R-factor	0.2352
Rwork	0.233
R-free	0.27800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.970
Data reduction software	autoPROC (1.0.5 (20200319))
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.070	2.000
High resolution limit [Å]	1.970	1.970
Rmerge	0.130
Number of reflections	18549	1004
<I/σ(I)>	12.2
Completeness [%]	92.4
Redundancy	19.1
CC(1/2)	0.991	0.396

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	9.5-11% PEG 3350 (v/v), 250-350 mM NaI, 100 mM Sodium Cacodylate [pH 6.5]. Crystals grew in 1 uL protein (3 mg/mL) + 1 uL crystallant. Cryoprotected in 20% (v/v) Glycerol.