9RP4

COLLAGENE_LIKE SEQUENCE (PPG)10 UNDER 1.4 GIGA PASCALS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID27
Synchrotron site	ESRF
Beamline	ID27
Temperature [K]	290
Detector technology	PIXEL
Collection date	2022-02-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.4099
Spacegroup name	P 21 21 21
Unit cell lengths	25.520, 25.820, 179.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.557 - 2.085
Rwork	0.258
R-free	0.33920
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.007
RMSD bond angle	1.764
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.557	2.150
High resolution limit [Å]	2.085	2.085
Rmerge		0.470
Number of reflections	6410	355
<I/σ(I)>	4
Completeness [%]	83.1
Redundancy	3.9
CC(1/2)	0.990	0.970

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	Vapor infusion of diglyme in well containing 10 muL of lyophilised (PPG)10 (5mg/mL in acetic acid 1O%)