9RO8

Pantoea ananatis encodes an antibacterial and anti-eukaryotic human CD38 homologue T6SS ADP-ribosyl cyclase polymorphic toxin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.82656
Spacegroup name	P 21 21 21
Unit cell lengths	44.230, 70.213, 96.119
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.660 - 1.590
R-factor	0.1847
Rwork	0.183
R-free	0.21570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.040
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.240	1.630
High resolution limit [Å]	1.590	1.590
Rmerge	0.092	1.053
Rmeas	0.096	1.099
Rpim	0.027	0.308
Number of reflections	40681	2765
<I/σ(I)>	17	2.2
Completeness [%]	99.5	92.7
Redundancy	13.2	11.9
CC(1/2)	0.999	0.758

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 6000 (20 % w/v), pH 5.0, 0.1 M Sodium acetate, 0.2 M Ammonium chloride