9RMD

Human MINDY3 deubiquitinase (FAM188A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-11
Detector	DECTRIS PILATUS3 S 2M
Wavelength(s)	0.9184
Spacegroup name	P 32 2 1
Unit cell lengths	112.800, 112.800, 72.080
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.420 - 2.300
R-factor	0.2398
Rwork	0.238
R-free	0.26430
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.872
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.360
High resolution limit [Å]	2.300	2.300
Number of reflections	23779	23705
<I/σ(I)>	12.04
Completeness [%]	99.5	96.5
Redundancy	8.1	6.8
CC(1/2)	0.999	0.461

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293.15	20% (v/v) PEG 6000 and MES pH 6.0