Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.96546 |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.590, 71.650, 131.460 |
| Unit cell angles | 102.57, 96.38, 90.01 |
Refinement procedure
| Resolution | 48.321 - 2.750 |
| Rwork | 0.214 |
| R-free | 0.26450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.690 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.820 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.053 | 0.335 |
| Rmeas | 0.075 | 0.473 |
| Number of reflections | 40627 | 1999 |
| <I/σ(I)> | 10.01 | |
| Completeness [%] | 90.9 | 60.7 |
| Redundancy | 1.7 | 1.5 |
| CC(1/2) | 0.996 | 0.739 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1M HEPES, 10% w/v PEG 6000 |
