9RJV

W-formate dehydrogenase from Nitratidesulfovibrio vulgaris (Desulfovibrio vulgaris) - aerobic soaked with 55 bar oxygen

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-02
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.8731
Spacegroup name	P 21 21 21
Unit cell lengths	64.837, 128.376, 148.809
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.651 - 1.830
Rwork	0.195
R-free	0.24120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.495
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.680	1.860
High resolution limit [Å]	1.830	1.830
Number of reflections	109987	5266
<I/σ(I)>	5.8
Completeness [%]	99.8
Redundancy	5.5
CC(1/2)	0.992	0.204

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	22% PEG 3350, 0.1M Tris-HCl pH 8.0, 1M LiCl