9RJ6
Zn Finger DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 292 |
| Detector technology | PIXEL |
| Collection date | 2024-01-14 |
| Detector | RIGAKU HyPix-Arc 150 |
| Wavelength(s) | 1.3405 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 44.449, 56.159, 129.793 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.380 - 1.950 |
| R-factor | 0.197 |
| Rwork | 0.195 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.908 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.380 | 2.150 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.100 | 0.459 |
| Rmeas | 0.107 | 0.490 |
| Rpim | 0.037 | 0.169 |
| Number of reflections | 15456 | 3811 |
| <I/σ(I)> | 14.33 | 5.16 |
| Completeness [%] | 99.8 | |
| Redundancy | 8.4 | |
| CC(1/2) | 0.997 | 0.945 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 292 | 0.05M Ca Acetate 0.05M MES pH=6 7.5% PEG 400 |
