9RIF

The histone fold domain heterodimer of oocyst rupture proteins 1 and 2 from Plasmodium berghei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-23
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.87313
Spacegroup name	P 21 21 21
Unit cell lengths	82.665, 112.922, 145.241
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	66.700 - 3.100
R-factor	0.2747
Rwork	0.274
R-free	0.29500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.841
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.21.2_5419))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.700	3.300
High resolution limit [Å]	3.100	3.100
Rmerge	0.175	1.295
Rmeas	0.189	1.395
Number of reflections	25331	4518
<I/σ(I)>	12	2.3
Completeness [%]	99.8	99.8
Redundancy	13.2
CC(1/2)	0.997	0.849

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	25% (w/v) PEG 3500, 0.2 M lithium sulphate (LiSO4) and 0.1 M HEPES pH 7.5