9RI6
Zn finger-finger DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 292 |
| Detector technology | PIXEL |
| Collection date | 2024-05-01 |
| Detector | RIGAKU HyPix-Arc 150 |
| Wavelength(s) | 1.3405 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 44.775, 55.514, 129.882 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.430 - 2.000 |
| R-factor | 0.186 |
| Rwork | 0.184 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.930 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.430 | 2.200 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.073 | 0.237 |
| Rmeas | 0.078 | 0.257 |
| Rpim | 0.028 | 0.098 |
| Number of reflections | 11290 | 2772 |
| <I/σ(I)> | 19.11 | 6.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.2 | 6.7 |
| CC(1/2) | 0.998 | 0.953 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 0.05M Mg Acetate 0.05M Na cacodylate 7.5% PEG 6000 |
