9RHP
Self-complemented Archaeal bundling protein X (AbpX)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-05-23 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 55.453, 55.453, 268.594 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.020 - 1.490 |
| R-factor | 0.2056 |
| Rwork | 0.203 |
| R-free | 0.24970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.540 |
| Data reduction software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.21_5207: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.024 | 5.966 |
| High resolution limit [Å] | 1.490 | 1.491 |
| Rpim | 0.015 | 0.307 |
| Number of reflections | 16094 | 805 |
| <I/σ(I)> | 18.6 | |
| Completeness [%] | 91.4 | |
| Redundancy | 34.2 | |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.16 M Calcium acetate hydrate 0.08 M Sodium cacodylate pH6.5 14.4 % w/v PEG 8000 20 % v/v Glycerol |
