9RGL
Crystal structure of the SroF-Sar complex, repressor and antirepressor of phage phi3T.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.942, 80.983, 157.971 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.530 - 3.050 |
| R-factor | 0.2423 |
| Rwork | 0.240 |
| R-free | 0.28210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.439 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 157.970 | 3.260 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.120 | 0.987 |
| Rpim | 0.040 | 0.449 |
| Number of reflections | 19883 | 3514 |
| <I/σ(I)> | 12 | |
| Completeness [%] | 99.8 | |
| Redundancy | 9.6 | |
| CC(1/2) | 0.998 | 0.933 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Imidazole pH 8, 30 % PEG 8000 and 200 mM NaC |
