9RGK

Human Transthyretin I107V Mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU PhotonJet-R
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-01
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	42.743, 85.814, 63.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.720 - 1.700
R-factor	0.1895
Rwork	0.188
R-free	0.21750
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.007
RMSD bond angle	0.853
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	2.030	1.740
High resolution limit [Å]	1.700	1.960	1.700
Rmeas	0.083	0.317	1.406
Number of reflections	25777	1621	1811
<I/σ(I)>	15.57	6.86	1.52
Completeness [%]	97.1	97.4	93.2
Redundancy	6.95	5.98	5.42
CC(1/2)	0.998	0.924	0.452

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	2M (NH4)2SO4; 100 mM citrate buffer pH 5.5; 7 v/v % glycerol