9RFM
Crystal structure of the metallo-beta-lactamase VIM-1 with 1760
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-01-24 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.78379 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.398, 67.771, 40.118 |
| Unit cell angles | 90.00, 93.17, 90.00 |
Refinement procedure
| Resolution | 40.060 - 1.050 |
| R-factor | 0.1134 |
| Rwork | 0.112 |
| R-free | 0.13240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.298 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (9.0.003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.770 | 1.070 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Number of reflections | 96446 | 4716 |
| <I/σ(I)> | 10.8 | |
| Completeness [%] | 98.6 | 98.1 |
| Redundancy | 6.9 | |
| CC(1/2) | 0.822 | 0.822 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 2.7M Ammonium Phosphate, 0.1M Tris |
