9RDO
SUDV VP40 in complex with 4-Fluoro-2-hydroxybenzamide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.999998 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 63.039, 90.722, 48.157 |
| Unit cell angles | 90.00, 95.10, 90.00 |
Refinement procedure
| Resolution | 47.970 - 1.650 |
| R-factor | 0.2373 |
| Rwork | 0.236 |
| R-free | 0.25620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.981 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.970 | 1.709 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 31936 | 3179 |
| <I/σ(I)> | 7.02 | |
| Completeness [%] | 98.4 | 97.93 |
| Redundancy | 6.4 | 6 |
| CC(1/2) | 0.986 | 0.842 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM HEPES; 40 mM MgCl2; 10% (v/v) PEG400; 20% ethylen glycol as cryoprotectant |
