9RDM
SUDV VP40 in complex with 3-aminosalicylic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.999998 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 62.741, 90.640, 48.313 |
| Unit cell angles | 90.00, 94.33, 90.00 |
Refinement procedure
| Resolution | 34.130 - 1.650 |
| R-factor | 0.1947 |
| Rwork | 0.193 |
| R-free | 0.21890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.873 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.130 | 1.536 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Number of reflections | 31769 | 3883 |
| <I/σ(I)> | 12.66 | |
| Completeness [%] | 98.0 | 86.42 |
| Redundancy | 3.2 | 2.4 |
| CC(1/2) | 0.998 | 0.511 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM HEPES; 40 mM MgCl2; 10% (v/v) PEG400; 20% ethylen glycol as cryoprotectant |
