9RDL
SUDV VP40 in complex with 4-fluorosalicylic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.999998 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 62.988, 90.781, 48.127 |
| Unit cell angles | 90.00, 95.00, 90.00 |
Refinement procedure
| Resolution | 45.390 - 1.750 |
| R-factor | 0.2052 |
| Rwork | 0.204 |
| R-free | 0.23010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.816 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.390 | 1.814 |
| High resolution limit [Å] | 1.750 | 1.751 |
| Number of reflections | 26780 | 2638 |
| <I/σ(I)> | 9.44 | |
| Completeness [%] | 98.5 | 98.03 |
| Redundancy | 3.8 | 3.8 |
| CC(1/2) | 0.998 | 0.395 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM HEPES, 40 mM MgCl2, 10% (v/v) PEG400, 20% ethylen glycol as cryoprotectant |
