9RDI

Crystal Structure of Flap Endonuclease FEN1 with Compound 5

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-04
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98011
Spacegroup name	P 21 21 21
Unit cell lengths	61.453, 116.150, 139.706
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.410 - 2.105
R-factor	0.2123
Rwork	0.211
R-free	0.23190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.210
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	BUSTER (2.11.8 (10-JUL-2024))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.075	2.326
High resolution limit [Å]	2.105	2.105
Rmerge	0.179
Rmeas	0.195
Rpim	0.076
Number of reflections	39509	1975
<I/σ(I)>	8.2
Completeness [%]	67.2
Redundancy	6.8
CC(1/2)	0.983	0.502

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	21 % PEG MME 5000, 0.1 M MES pH 6.5, 10 mM ammonium sulphate