9RCU

Cardioderma bat coronavirus 2B receptor binding domain in complex with human CEACAM6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-05-11
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.954
Spacegroup name	P 21 21 21
Unit cell lengths	47.553, 76.856, 204.975
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.490 - 2.990
R-factor	0.255
Rwork	0.253
R-free	0.29730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.912
Data reduction software	DIALS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.490	3.310
High resolution limit [Å]	2.989	2.989
Rmerge	0.226	1.392
Rmeas	0.237	1.451
Rpim	0.068	0.403
Number of reflections	9386	486
<I/σ(I)>	6.4	1.6
Completeness [%]	59.2
Redundancy	12.2
CC(1/2)	0.974	0.844

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	Protein complex was 1:1.2 molar ratio CEACAM6:CcCoV-2B RBD, 2.7 mg/mL, in 10 mM Tris pH 7.5, 150 mM NaCl. 200 nL protein was incubated with 200 nL reservoir against an 80 uL reservoir containing 0.1 M SPG buffer system pH 5, 25% w/v PEG 1500.