9RCS

Cardioderma bat coronavirus KY43 receptor binding domain in complex with human CEACAM6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-02-23
Detector	DECTRIS EIGER2 X CdTe 9M
Wavelength(s)	0.620
Spacegroup name	P 1 21 1
Unit cell lengths	70.010, 78.795, 82.339
Unit cell angles	90.00, 88.64, 90.00

Refinement procedure

Resolution	82.320 - 3.012
R-factor	0.2584
Rwork	0.256
R-free	0.31680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.071
Data reduction software	DIALS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.332	3.388
High resolution limit [Å]	3.012	3.012
Rmerge	0.196	1.259
Rmeas	0.214	1.370
Rpim	0.085	0.537
Number of reflections	8711	439
<I/σ(I)>	5.4	1.6
Completeness [%]	48.5	8.3
Redundancy	6.3
CC(1/2)	0.994	0.583

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.3	293	Protein complex was 1:1.2 molar ratio CEACAM6:KY43-RBD, 2 mg/mL, in 10 mM Tris pH 7.5, 150 mM NaCl). 200 nL protein was incubated with 200 nL reservoir against an 80 uL reservoir containing 0.1 M Na acetate pH 4.3, 21.5% PEG 5K MME, 5% glycerol.