9RC7
Crystal structure of Gilliamella bombicola lactate deydrogenase (GbLDH)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-09-26 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9655 |
| Spacegroup name | P 1 |
| Unit cell lengths | 93.229, 93.559, 192.842 |
| Unit cell angles | 100.42, 91.82, 90.19 |
Refinement procedure
| Resolution | 49.240 - 2.200 |
| R-factor | 0.23093 |
| Rwork | 0.230 |
| R-free | 0.24785 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.545 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.240 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.161 | 0.358 |
| Rmeas | 0.194 | 0.440 |
| Rpim | 0.105 | 0.251 |
| Total number of observations | 999841 | 25590 |
| Number of reflections | 301715 | 8858 |
| <I/σ(I)> | 6.3 | 1.4 |
| Completeness [%] | 92.8 | |
| Redundancy | 3.3 | 2.9 |
| CC(1/2) | 0.871 | 0.211 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.1 M Tris pH 7.0 -7.5 10-12% PEG3350 and 10% glycerol |
