9RC4
Crystal structure of VirJ domain 1 from Brucella
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.638, 47.235, 54.124 |
| Unit cell angles | 80.92, 89.06, 86.14 |
Refinement procedure
| Resolution | 38.200 - 1.740 |
| R-factor | 0.1805 |
| Rwork | 0.179 |
| R-free | 0.20560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.786 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.540 | 1.770 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.107 | 0.845 |
| Rmeas | 0.126 | 0.990 |
| Rpim | 0.066 | 0.514 |
| Total number of observations | 143534 | 7771 |
| Number of reflections | 39671 | 2139 |
| <I/σ(I)> | 4.8 | 1.2 |
| Completeness [%] | 96.9 | |
| Redundancy | 3.6 | 3.6 |
| CC(1/2) | 0.994 | 0.544 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 292 | PEG 3350 16% w/v, 0.06M citric acid pH 4.1, 0.04M BIS-TRIS propane |
