9RBZ
DvhD, HD-GYP domain, c-di-GMP-bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-08-28 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.927, 44.939, 115.605 |
| Unit cell angles | 89.89, 101.41, 96.21 |
Refinement procedure
| Resolution | 43.770 - 1.800 |
| R-factor | 0.1921 |
| Rwork | 0.191 |
| R-free | 0.22850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.641 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.800 | 1.820 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 137047 | 2476 |
| <I/σ(I)> | 9.5 | |
| Completeness [%] | 84.0 | |
| Redundancy | 1.9 | |
| CC(1/2) | 0.993 | 0.959 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M ammonium acetate, 0.1 M Bis-Tris pH 6.5, 25% (w/v) polyethylene glycol 3,350, and 20% xylitol |
