9RAN

Structural characterization of a galactose-binding site in human RNase2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	41.667, 52.668, 56.452
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.510 - 1.020
R-factor	0.1394
Rwork	0.139
R-free	0.15170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.832
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.510	1.040
High resolution limit [Å]	1.016	1.020
Number of reflections	54606	2730
<I/σ(I)>	7.53
Completeness [%]	84.3
Redundancy	3.9
CC(1/2)	0.994	0.991

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.18M Sodium Formate, 0.1M Bis-Tris propane 15% PEG 3350 ph=8.2