9R9L

sucrose hydrolase SuxB from Xanthomonas oryzae pv. oryzae in complex with glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-03-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.059700
Spacegroup name	I 2 2 2
Unit cell lengths	100.535, 172.726, 259.777
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.750 - 2.950
R-factor	0.23277
Rwork	0.229
R-free	0.30182
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.088
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.750	3.055
High resolution limit [Å]	2.950	2.950
Number of reflections	47939	4747
<I/σ(I)>	11.07
Completeness [%]	100.0
Redundancy	13.2
CC(1/2)	0.998	0.880

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		285	0.25 M potassium sodium tartrate, 37.5 (w/v) % PEG 3350