9R9C

Crystal structure of TRIM24 PHD-bromodomain with XS839112

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-01-20
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97627
Spacegroup name	C 1 2 1
Unit cell lengths	89.110, 36.823, 129.419
Unit cell angles	90.00, 110.10, 90.00

Refinement procedure

Resolution	60.800 - 1.590
R-factor	0.150536674579
Rwork	0.148
R-free	0.19569
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.006
RMSD bond angle	0.808
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.800	1.620
High resolution limit [Å]	1.590	1.590
Rmerge	0.081	0.939
Number of reflections	52968	2610
<I/σ(I)>	12.7	2
Completeness [%]	98.9	98.2
Redundancy	7	7.1
CC(1/2)	0.999	0.821

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	Protein solution: 11 mg/ml protein in 150 mM NaCl, 25 mM HEPES, pH 7.5, 0.5 mM TCEP, 5% glycerol. Reservoir solution: 3% PEG 400, 2.6 M ammonium sulfate, 0.1 M HEPES pH 7.5 (protein:buffer 1:2). Crystals soaked with 0.1 mM compound in reservoir buffer complemented with 25% glycerol.