9R8I

sucrose hydrolase SuxB from Xanthomonas oryzae pv. oryzae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-01-25
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.873130
Spacegroup name	I 2 2 2
Unit cell lengths	98.754, 175.959, 258.335
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.980 - 2.400
R-factor	0.1694
Rwork	0.166
R-free	0.22820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.277
Data reduction software	autoPROC
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.980	2.393
High resolution limit [Å]	2.310	2.310
Number of reflections	98585	9735
<I/σ(I)>	7.07
Completeness [%]	100.0
Redundancy	6.9
CC(1/2)	0.991	0.626

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		285	0.25 M potassium sodium tartrate, 32.5 (w/v) % PEG 3350