9R84

The C103F Mutant of Apo-C-Terminal Domain Homolog of the Orange Carotenoid Protein (CTDH) from Anabaena

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9655
Spacegroup name	C 1 2 1
Unit cell lengths	106.532, 63.914, 48.565
Unit cell angles	90.00, 99.46, 90.00

Refinement procedure

Resolution	37.640 - 1.750
R-factor	0.2359
Rwork	0.235
R-free	0.25950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.066
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.640	1.780
High resolution limit [Å]	1.750	1.750
Number of reflections	31011	1684
<I/σ(I)>	20.8
Completeness [%]	95.1
Redundancy	2.5
CC(1/2)	0.998	0.933

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	37% Tacsimate