9R7J
Repair of Iron Centre (RIC) protein from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 158.219, 111.105, 122.634 |
| Unit cell angles | 90.00, 93.93, 90.00 |
Refinement procedure
| Resolution | 55.550 - 2.730 |
| R-factor | 0.2642 |
| Rwork | 0.263 |
| R-free | 0.29570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.322 |
| Data reduction software | XDS (20170615) |
| Data scaling software | STARANISO (1.0.4) |
| Phasing software | PHASER (2.7.17) |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.560 | 2.990 |
| High resolution limit [Å] | 2.730 | 2.730 |
| Rmerge | 0.092 | 0.788 |
| Rmeas | 0.930 | |
| Rpim | 0.059 | 0.488 |
| Number of reflections | 38819 | 1942 |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 92.5 | 67.6 |
| Redundancy | 3.3 | 3.6 |
| CC(1/2) | 0.998 | 0.494 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M Na-HEPES pH7.5, 0.6M NaH2PO4, 0.6M KH2PO4 |
