9R7B
Structure and mechanism of the broad spectrum CRISPR-associated ring nuclease Crn4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-10-02 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 46.340, 61.330, 42.670 |
| Unit cell angles | 90.00, 101.95, 90.00 |
Refinement procedure
| Resolution | 30.665 - 1.100 |
| Rwork | 0.168 |
| R-free | 0.18520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.295 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.680 | 1.110 |
| High resolution limit [Å] | 1.090 | 1.090 |
| Rmerge | 0.036 | 4.830 |
| Number of reflections | 38596 | 250 |
| <I/σ(I)> | 26 | |
| Completeness [%] | 79.4 | |
| Redundancy | 11.9 | |
| CC(1/2) | 1.000 | 0.228 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Calcium chloride 20% PEG 6000 10% ethylene glycol |
