9R74
Pyrin B30.2 domain, FMF mutation I641F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-04-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 60.127, 60.127, 180.698 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.100 - 2.000 |
| R-factor | 0.21101 |
| Rwork | 0.207 |
| R-free | 0.27644 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.674 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.120 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.222 | 1.141 |
| Rmeas | 0.260 | 1.351 |
| Rpim | 0.133 | 0.710 |
| Total number of observations | 3222 | |
| Number of reflections | 13775 | 970 |
| <I/σ(I)> | 4.6 | 1 |
| Completeness [%] | 99.3 | |
| Redundancy | 3.5 | 3.3 |
| CC(1/2) | 0.981 | 0.355 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Tris-AcOH, pH 7.6; 0.2 M KSCN; 20 % (w/v) PEG 2k MME |
