9R6D

Structural characterization of a trehalose-binding site in human RNase2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	42.002, 52.527, 56.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.560 - 0.940
R-factor	0.1392
Rwork	0.139
R-free	0.14790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.849
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.560	0.970
High resolution limit [Å]	0.940	0.940
Number of reflections	72600	1012
<I/σ(I)>	1.93
Completeness [%]	90.3
Redundancy	5.3
CC(1/2)	0.995	0.835

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1M Sodium formate, 14% PEG 3350, PH=7.2