9R5L
Crystal structure of class Ie ribonucleotide reductase R2 subunit from Mesoplasma florum with a D212N mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-29 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 141.083, 45.774, 56.833 |
| Unit cell angles | 90.00, 112.22, 90.00 |
Refinement procedure
| Resolution | 43.200 - 1.700 |
| R-factor | 0.2133 |
| Rwork | 0.211 |
| R-free | 0.25670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.747 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.200 | 1.750 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.058 | 1.316 |
| Rmeas | 0.062 | 1.426 |
| Rpim | 0.023 | 0.534 |
| Number of reflections | 36454 | 2704 |
| <I/σ(I)> | 13.09 | 1.21 |
| Completeness [%] | 97.8 | 96.43 |
| Redundancy | 7 | 6.9 |
| CC(1/2) | 0.713 | 0.713 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 100 mM ammonium sulfate, 12-16% PEG 3350, 100-200 mM calcium acetate |
