9R3T

C-terminal domain of SdeA Legionella effector

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-09-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	H 3 2
Unit cell lengths	110.956, 110.956, 218.708
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	55.480 - 1.980
R-factor	0.225
Rwork	0.224
R-free	0.25040
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.765
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	87.970	2.030
High resolution limit [Å]	1.980	1.980
Number of reflections	97198	3992
<I/σ(I)>	23.9
Completeness [%]	100.0
Redundancy	9.9
CC(1/2)	1.000	0.586

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M sodium chloride, 0.1 M sodium/ potassium phosphate pH-7.5, 50% PEG 200