9R3P
Hemoglobin Glb2-1 of Lotus japonicus (cyano-ferric form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979263 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 35.928, 127.656, 152.733 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 76.370 - 1.600 |
| R-factor | 0.17978 |
| Rwork | 0.178 |
| R-free | 0.20558 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.926 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 152.730 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.050 | 0.434 |
| Number of reflections | 47061 | 6768 |
| <I/σ(I)> | 15.2 | 2.9 |
| Completeness [%] | 100.0 | |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 28% PEG 4K, 200 mM ammonium citrate, 4% polypropylene glycol, and 100 mM KCN |
