9R2Z
RptR transcriptional repressor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-04-13 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.603, 41.541, 63.261 |
| Unit cell angles | 90.00, 90.11, 90.00 |
Refinement procedure
| Resolution | 34.720 - 1.300 |
| R-factor | 0.1666 |
| Rwork | 0.165 |
| R-free | 0.19500 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.791 |
| Phasing software | PHASER |
| Refinement software | REFMAC (v5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.720 | 1.210 |
| High resolution limit [Å] | 1.190 | 1.190 |
| Rmerge | 0.074 | |
| Rmeas | 0.081 | |
| Rpim | 0.031 | |
| Number of reflections | 51246 | 2435 |
| <I/σ(I)> | 9.4 | 0.7 |
| Completeness [%] | 99.5 | 96.7 |
| Redundancy | 6.7 | 4.7 |
| CC(1/2) | 0.998 | 0.382 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 150 mM potassium thiocyanate, 32% PEG 2000 MME |
