9R11

Structure of the PRYSPRY domain of human MID2, crystal form II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	53.406, 60.919, 118.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.700 - 1.640
R-factor	0.157888917472
Rwork	0.155
R-free	0.20569
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.823
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.700	1.670
High resolution limit [Å]	1.640	1.640
Rmerge	0.070	1.054
Number of reflections	48227	2354
<I/σ(I)>	14.6	1.9
Completeness [%]	99.9
Redundancy	9
CC(1/2)	0.999	0.812

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Protein solution: 9 mg/ml MID2 PRYSPRY in 20 mM HEPES pH 7.5, 100 mM NaCl, and 0.5 mM TCEP. Reservoir solution: 7% PEG 6000, 5% MPD, 0.1 M HEPES, pH 7.0.