9R0K
Structure of the human heterotetrameric cis-prenyltransferase complex in its apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-08-06 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 183.346, 183.346, 112.356 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.013 - 2.890 |
| R-factor | 0.2668 |
| Rwork | 0.265 |
| R-free | 0.30210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.652 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.858 | 3.060 |
| High resolution limit [Å] | 2.890 | 2.890 |
| Rmeas | 0.055 | 1.388 |
| Number of reflections | 16334 | 2603 |
| <I/σ(I)> | 35.95 | |
| Completeness [%] | 100.0 | |
| Redundancy | 20.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 292 | 0.1 M LiCl, 0.05 M Glycine pH 9.5, 40% (v/v) PEG 400 |
